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Bacillus thuringiensis sporulation at suboptimal temperature.

J N Aronson, F M Thompson

    Journal of Bacteriology
    |January 1, 1971
    PubMed
    Summary
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    Bacillus thuringiensis Berliner grown at 14°C showed slower spore and parasporal inclusion production. However, sporulation levels reached 50-100% compared to optimal 30°C growth.

    Area of Science:

    • Microbiology
    • Bacterial Physiology

    Background:

    • Bacillus thuringiensis is an entomopathogenic bacterium.
    • Spore and parasporal inclusion formation are critical for its biological activity.

    Purpose of the Study:

    • To investigate the effect of low temperature (14°C) on Bacillus thuringiensis var. thuringiensis sporulation.
    • To compare sporulation efficiency at 14°C with optimal growth temperatures.

    Main Methods:

    • Culturing Bacillus thuringiensis var. thuringiensis on a chemically defined liquid medium.
    • Incubation at a reduced temperature of 14°C.
    • Assessing spore and parasporal inclusion production.

    Main Results:

    • Slowed production of spores and parasporal inclusions at 14°C.

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  • Sporulation yield at 14°C reached 50-100% of that observed at 30°C.
  • Parasporal inclusion formation was also observed at the lower temperature.
  • Conclusions:

    • Low temperatures (14°C) significantly slow down Bacillus thuringiensis sporulation but do not completely inhibit it.
    • Adequate spore and parasporal inclusion yields can still be achieved at suboptimal temperatures, suggesting some resilience in the sporulation process.