Related Experiment Videos
Glycopeptides from the surface of control and virus-transformed cells
Science (New York, N.Y.)
|April 9, 1971
Abstract:
Glycopeptides were removed by trypsin digestion from the surface of control cells and cells transformed by Rous sarcoma virus, murine sarcoma virus, or polyoma virus. After digestion with pronase, the glycopeptides were analyzed by gel filtration. The elution profiles suggest that there are differences in the glycopeptides from the surface of control cells and those from transformed cells.
Insights
Surface glycopeptides differ between normal and virus-transformed cells. Trypsin digestion and pronase treatment revealed distinct glycopeptide profiles in control versus Rous sarcoma, murine sarcoma, and polyoma virus-infected cells.
Area of Science:
- Cell Biology
- Virology
- Glycobiology
Background:
- Cell surface glycoproteins play crucial roles in cell adhesion and communication.
- Viral transformation alters cellular processes, potentially affecting cell surface composition.
Purpose of the Study:
- To investigate potential alterations in cell surface glycopeptides following viral transformation.
- To compare glycopeptide profiles of normal cells with those transformed by Rous sarcoma virus, murine sarcoma virus, and polyoma virus.
Main Methods:
- Cell surface proteins were isolated from control and virus-transformed cells using trypsin digestion.
- Isolated glycopeptides were further digested with pronase.
- Glycopeptide analysis was performed using gel filtration chromatography.
Main Results:
- Distinct elution profiles were observed when comparing glycopeptides from control cells and virus-transformed cells.
- These differences suggest variations in the composition or structure of cell surface glycopeptides after viral transformation.
Conclusions:
- Viral transformation by Rous sarcoma virus, murine sarcoma virus, or polyoma virus leads to detectable changes in cell surface glycopeptides.
- Further characterization of these altered glycopeptides may elucidate their role in the transformed phenotype.