Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Semi-micro, dye-binding assay for rabbit interferon.

J A Armstrong

    Applied Microbiology
    |April 1, 1971
    PubMed
    Summary
    This summary is machine-generated.

    A new semi-micro interferon assay quantifies cytopathic effect inhibition for sensitive and economical viral detection. Optimizing culture age is crucial for reproducible results in this interferon detection method.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Retroviral infection of accessory cells and the immunological paradox in AIDS.

    Immunology today·2014
    Same author

    Oxidative stress in acute pancreatitis: lost in translation?

    Free radical research·2013
    Same author

    Water polarization induced by thermal gradients: the extended simple point charge model (SPC/E).

    The Journal of chemical physics·2013
    Same author

    Statistical mechanics of coarse graining: estimating dynamical speedups from excess entropies.

    The Journal of chemical physics·2012
    Same author

    Computational verification of two universal relations for simple ionic liquids. Kinetic properties of a model 2:1 molten salt.

    The journal of physical chemistry. B·2011
    Same author

    The susceptibility of adult female Anopheles gambiae to insecticides in East Africa.

    Bulletin of the World Health Organization·2010
    Same journal

    Separation of spores and parasporal crystals of Bacillus thuringiensis in gradients of certain x-ray contrasting agents.

    Applied microbiology·1975
    Same journal

    Microbial indicators of oil-rich salt marsh sediments.

    Applied microbiology·1975
    Same journal

    Accelerated Fermentation of Brewer's Wort by Saccharomyces carlsbergensis.

    Applied microbiology·1975
    Same journal

    Semisolid fermentation of ryegrass straw.

    Applied microbiology·1975
    Same journal

    Microbiology of ripening honey.

    Applied microbiology·1975
    Same journal

    Microwave sanitization of color additives used in cosmetics: feasibility study.

    Applied microbiology·1975
    See all related articles

    Area of Science:

    • Virology
    • Immunology
    • Biotechnology

    Background:

    • Interferon assays are vital for antiviral research and diagnostics.
    • Existing methods like plaque inhibition can be less sensitive or economical.
    • A need exists for a more efficient and sensitive interferon quantification technique.

    Purpose of the Study:

    • To introduce and validate a novel semi-micro method for interferon assay.
    • To evaluate the sensitivity and economy of this new method compared to traditional techniques.
    • To identify key factors influencing the reproducibility of the assay.

    Main Methods:

    • Utilized a semi-micro assay format in 6-mm wells.
    • Quantified inhibition of cytopathic effects induced by vesicular stomatitis virus.

    Related Experiment Videos

  • Employed colorimetric measurement of eluted methylrosanaline chloride dye.

    • Main Results:

    • The semi-micro method demonstrated higher sensitivity than the plaque-inhibition method.
    • The assay proved to be extremely economical.
    • Culture age was identified as the most significant factor impacting assay reproducibility.

    Conclusions:

    • The semi-micro interferon assay offers a sensitive, economical, and viable alternative for interferon quantification.
    • Further optimization, particularly regarding culture age, can enhance the reliability of this assay.
    • This method holds promise for widespread application in virology and immunology research.