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Plasma adenine and cellular ATP in red cell concentrates collected and stored in modified CPD at 4 C
Insights
Preparing red blood cell concentrates (RCC) depletes adenine, accelerating adenosine triphosphate (ATP) loss. Higher anticoagulant adenine levels are recommended for extended storage or higher hematocrit (Hct) RCC.
Area of Science:
- Biomedical Science
- Blood Preservation
- Hematology
Background:
- Optimizing red blood cell concentrate (RCC) storage is crucial for transfusion efficacy.
- Anticoagulant adenine plays a role in maintaining red blood cell viability during storage.
- Understanding adenine and adenosine triphosphate (ATP) dynamics in RCC is essential.
Purpose of the Study:
- To investigate the impact of red blood cell concentrate preparation on adenine availability.
- To assess the rate of adenosine triphosphate (ATP) loss in RCC compared to whole blood.
- To determine optimal anticoagulant adenine levels for extended RCC storage.
Main Methods:
- Blood units were collected in modified citrate-phosphate-dextrose (CPD) with increased glucose and adenine.
- Red blood cell concentrates (RCC) were prepared to a mean hematocrit (Hct) of 70%.
- Plasma adenine and red blood cell adenosine triphosphate (ATP) levels were measured weekly for 42 days at 4°C.
Main Results:
- Plasma adenine was reduced by 39% due to plasma removal during RCC preparation.
- Measurable plasma adenine was depleted by 21 days of storage.
- Adenosine triphosphate (ATP) loss in RCC occurred significantly faster than in whole blood.
Conclusions:
- Plasma removal during RCC preparation significantly impacts adenine availability.
- Extended storage or higher Hct in RCC accelerates ATP degradation.
- Increased anticoagulant adenine supplementation is advised for prolonged RCC storage or higher Hct preparations.
Abstract:
Eight units of blood were drawn into modified CPD containing 25 per cent higher glucose and 17.3 mg adenine (0.25 mM in blood). Red blood cell concentrates (RCC) were prepared to a mean hematocrit (Hct) of 70, the cells stored at 4 C, and plasma adenine and red blood cell adenosine triphosphate (ATP) were measured weekly for 42 days. The removal of plasma in the preparation of RCC reduced by 39 per cent the available adenine. As a result measurable plasma adenine was depleted by 21 days. The loss of ATP in RCC occurs at a significantly faster rate than in whole blood stored under the same conditions. When red blood cells are stored at higher HCT or for periods longer than 35 days, increased anticoagulant adenine levels are recommended.