Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enterovirus concentration on cellulose membranes.

C Wallis, M Henderson, J L Melnick

    Applied Microbiology
    |March 1, 1972
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Development of an in-home screening tool for canine periodontitis.

    Veterinary journal (London, England : 1997)·2025
    Same author

    Persistent deciduous teeth: Association of prevalence with breed, breed size and body weight in pure-bred client-owned dogs in the United States.

    Research in veterinary science·2024
    Same author

    Scintillation light detection in the 6-m drift-length ProtoDUNE Dual Phase liquid argon TPC.

    The European physical journal. C, Particles and fields·2022
    Same author

    The feasibility of home monitoring of young people with cystic fibrosis: Results from CLIMB-CF.

    Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2021
    Same author

    Association of periodontal disease with breed size, breed, weight, and age in pure-bred client-owned dogs in the United States.

    Veterinary journal (London, England : 1997)·2021
    Same author

    Subgingival microbiota of dogs with healthy gingiva or early periodontal disease from different geographical locations.

    BMC veterinary research·2021

    Concentrating viruses from water is efficient using cellulose nitrate membranes with trivalent salts like aluminum chloride (AlCl3). This method significantly reduces salt concentration and volume needed for effective virus adsorption and elution.

    Area of Science:

    • Environmental microbiology
    • Water quality analysis
    • Virology

    Background:

    • Effective concentration of viruses from large water volumes is crucial for accurate detection and monitoring.
    • Traditional methods often require large amounts of chemicals and complex procedures.
    • Cellulose nitrate membranes offer a potential substrate for virus adsorption.

    Purpose of the Study:

    • To evaluate the efficacy of cellulose nitrate membranes for concentrating viruses from water.
    • To determine the role of salt valency in enhancing virus adsorption to these membranes.
    • To develop an efficient method for virus elution and subsequent concentration for assay.

    Main Methods:

    • Utilized cellulose nitrate membranes as adsorbents for virus concentration.

    Related Experiment Videos

  • Investigated the effect of different salt valencies (divalent and trivalent) on virus adsorption efficiency.
  • Optimized salt concentration, using aluminum chloride (AlCl3) as a trivalent salt example.
  • Developed a two-step elution and re-adsorption process using pH adjustment and AlCl3.
  • Assayed concentrated virus samples.

    • Main Results:

    • Salt addition was necessary for virus adsorption onto cellulose nitrate membranes.
    • Trivalent salts, such as AlCl3, were significantly more effective than divalent salts (e.g., MgCl2) at lower concentrations.
    • A concentration of 0.5 mM AlCl3 was as effective as 50 mM MgCl2 for virus adsorption.
    • The method allowed for the concentration of viruses from 500 gallons of water using minimal AlCl3.
    • A two-step elution and re-adsorption process efficiently concentrated viruses onto a smaller membrane for assay.

    Conclusions:

    • Cellulose nitrate membranes, in conjunction with trivalent salts, provide an effective and economical method for concentrating viruses from large volumes of water.
    • The developed procedure significantly reduces the amount of salt required compared to using divalent salts.
    • This technique offers a basis for concentrating minute virus quantities for sensitive detection and analysis in water samples.