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Yeast membrane vesicles: isolation and general characteristics.

M S Christensen, V P Cirillo

    Journal of Bacteriology
    |June 1, 1972
    PubMed
    Summary
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    Researchers isolated yeast membrane vesicles using manual grinding and centrifugation. These vesicles exhibit osmotic sensitivity and energy-independent transport, indicating potential for studying yeast cell membrane functions.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Yeast Research

    Background:

    • Understanding yeast cell membrane properties is crucial for various biological and biotechnological applications.
    • Previous methods for isolating yeast membrane vesicles were often complex or inefficient.

    Purpose of the Study:

    • To develop and characterize a method for isolating functional yeast membrane vesicles.
    • To assess the properties and potential enzymatic markers of these isolated vesicles.

    Main Methods:

    • Yeast cells were manually ground with glass beads (0.2 mm) in a porcelain mortar.
    • Vesicles were separated using differential centrifugation and elution from a glass bead column.
    • Osmotic sensitivity, cytoplasmic component presence, and transport functions were analyzed.

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    Main Results:

    • Isolated yeast membrane vesicles demonstrated osmotic sensitivity and contained cytoplasmic components.
    • Vesicles exhibited energy-independent transport function.
    • Glucose metabolism was absent, but respiratory function, likely due to intravesicular mitochondria, was observed.

    Conclusions:

    • A viable method for isolating functional yeast membrane vesicles was established.
    • The isolated vesicles possess key characteristics suitable for studying membrane transport and integrity.
    • Invertase and oligomycin-insensitive ATPase were identified as potential markers for lysed yeast vesicle preparations.