Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Specific initiation site for simian virus 40 deoxyribonucleic acid replication.

M M Thoren, E D Sebring, N P Salzman

    Journal of Virology
    |September 1, 1972
    PubMed
    Summary

    Researchers identified a specific origin for simian virus 40 (SV40) deoxyribonucleic acid (DNA) replication. This finding indicates that SV40 DNA replication is not random but starts at a single, defined site.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Parallel evolution of drug resistance in HIV: failure of nonsynonymous/synonymous substitution rate ratio to detect selection.

    Molecular biology and evolution·1999
    Same author

    Drug resistance during indinavir therapy is caused by mutations in the protease gene and in its Gag substrate cleavage sites.

    Journal of virology·1997
    Same author

    Emergence of protease inhibitor resistance mutations in human immunodeficiency virus type 1 isolates from patients and rapid screening procedure for their detection.

    Antimicrobial agents and chemotherapy·1996
    Same author

    Combination therapy with didanosine and interferon-alpha in human immunodeficiency virus-infected patients: results of a phase I/II trial.

    The Journal of infectious diseases·1996
    Same author

    Increases in CD4 T lymphocytes with intermittent courses of interleukin-2 in patients with human immunodeficiency virus infection. A preliminary study.

    The New England journal of medicine·1995
    Same author

    Application of branched DNA signal amplification to monitor human immunodeficiency virus type 1 burden in human plasma.

    The Journal of infectious diseases·1994

    Area of Science:

    • Molecular Biology
    • Virology
    • Genetics

    Background:

    • Simian virus 40 (SV40) deoxyribonucleic acid (DNA) replication is a complex process.
    • Understanding the initiation of viral DNA replication is crucial for comprehending viral life cycles and developing antiviral strategies.

    Purpose of the Study:

    • To investigate the mechanism of SV40 DNA replication initiation.
    • To determine if SV40 DNA replication begins at a specific site or randomly.

    Main Methods:

    • Isolation of replicating SV40 DNA molecules labeled with (3)H-thymidine.
    • Alkaline treatment to release newly synthesized DNA strands.
    • Analysis of single-stranded SV40 DNA fragments of varying sizes.
    • Measurement of duplex formation rates (renaturation kinetics) to assess genetic complexity.

    Main Results:

    • Newly synthesized SV40 DNA strands were isolated, ranging in size from 157,000 to 1,360,000 daltons.
    • The rate of renaturation of newly synthesized DNA increased proportionally with the extent of replication.
    • Data indicated a single, specific initiation site for SV40 DNA replication.

    Conclusions:

    • SV40 DNA replication is initiated at a unique, specific site.
    • Replication does not initiate randomly, suggesting a regulated process.

    Related Experiment Videos