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Related Experiment Videos

Bidirectional replication of Simian Virus 40 DNA.

K J Danna, D Nathans

    Proceedings of the National Academy of Sciences of the United States of America
    |November 1, 1972
    PubMed
    Summary

    Simian Virus 40 (SV40) DNA replication initiates at a specific site and proceeds bidirectionally. This study determined the temporal order of DNA fragment synthesis, revealing a bidirectional replication pattern for SV40.

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    Area of Science:

    • Molecular Biology
    • Virology
    • Genetics

    Background:

    • Simian Virus 40 (SV40) is a well-characterized DNA virus used extensively in molecular biology research.
    • Understanding viral DNA replication mechanisms provides insights into fundamental cellular processes.

    Purpose of the Study:

    • To elucidate the mechanism and directionality of SV40 DNA replication.
    • To determine the origin and termination sites of SV40 DNA synthesis.

    Main Methods:

    • Pulse-labeling of SV40 DNA with radioactive thymidine in infected monkey cells.
    • Analysis of DNA fragments generated by restriction endonuclease digestion (Hemophilus influenzae).
    • Deduction of the temporal order of fragment synthesis from labeled DNA distribution.

    Main Results:

    • A temporal order of fragment synthesis was established.
    • Comparison with the physical map revealed correspondence for specific fragment groups.
    • Evidence suggests replication initiates at a unique site on the SV40 genome.

    Conclusions:

    • SV40 DNA replication proceeds bidirectionally from a specific origin.
    • Replication terminates approximately halfway around the circular DNA molecule.
    • The findings support a model of semi-conservative, bidirectional DNA replication for SV40.

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