Adenovirus susceptibility to human interferon during one-step replication.

Area of Science:

• Virology
• Immunology
• Cell Biology

Background:

• Human interferons are crucial antiviral proteins.
• Adenoviruses are common human pathogens.
• Vesicular stomatitis virus (VSV) is a model virus for studying interferon responses.

Purpose of the Study:

• To assess the susceptibility of adenovirus types 2, 7, and 12 to human interferon.
• To compare interferon susceptibility between adenoviruses and VSV in human diploid cell strains.

Main Methods:

• Single-cycle infection of three human diploid cell strains with adenovirus types 2, 7, and 12.
• Measurement of viral yield at varying human interferon doses.
• Comparative analysis of adenovirus and VSV inhibition by interferon.

Main Results:

• Adenovirus yield decreased with increasing interferon doses across all tested cell strains.
• Relative susceptibility of adenovirus types to interferon differed among cell strains.
• High-dose interferon (>100 units) significantly inhibited VSV but had minimal effect on adenoviruses.

Conclusions:

• Adenoviruses exhibit variable, but generally lower, susceptibility to human interferon compared to VSV.
• Interferon efficacy against adenoviruses is dose-dependent and influenced by the host cell type.
• These findings highlight differential antiviral responses to interferon among distinct viral agents.