Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interaction of complement and polymyxin with gram-negative bacteria.

H Pruul, B L Reynolds

    Infection and Immunity
    |November 1, 1972
    PubMed
    Summary

    Complement and polymyxin work together to kill gram-negative bacteria like Salmonella typhimurium. They damage bacterial membranes, allowing other agents to cause cell death.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Uptake of cefepime by phagocytosing polymorphonuclear neutrophils and subsequent intracellular killing.

    Antimicrobial agents and chemotherapy·1996
    Same author

    Cefdinir-induced modification of the susceptibility of bacteria to the antibacterial activity of human serum and polymorphonuclear neutrophils.

    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·1993
    Same author

    Potentiation of azithromycin activity against Escherichia coli by human serum ultrafiltrate.

    The Journal of antimicrobial chemotherapy·1992
    Same author

    Potentiation of antibacterial activity of azithromycin and other macrolides by normal human serum.

    Antimicrobial agents and chemotherapy·1992
    Same author

    Macrolides and the immune system.

    Scandinavian journal of infectious diseases. Supplementum·1992
    Same author

    Phagocyte uptake and transport of azithromycin.

    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·1991

    Area of Science:

    • Microbiology
    • Immunology
    • Bacteriology

    Background:

    • Gram-negative bacteria possess a complex cell envelope.
    • The complement system and polymyxin are known antibacterial agents.
    • Understanding their precise mechanisms is crucial for developing new therapies.

    Purpose of the Study:

    • To elucidate the interaction sites of complement and polymyxin on gram-negative bacteria.
    • To investigate the synergistic bactericidal effects of complement and polymyxin.
    • To determine the sequence of membrane damage leading to bacterial cell death.

    Main Methods:

    • Utilized three strains of Salmonella typhimurium for experiments.
    • Assessed the impact of various test system components on bactericidal activity.
    • Analyzed the synergistic action between complement and polymyxin.

    Main Results:

    • Complement and polymyxin exhibit similar sensitivities to test system variations.
    • Synergistic bactericidal activity was observed between complement and polymyxin.
    • Evidence suggests both agents create lesions in the outer bacterial membrane.
    • Lysozyme access to its substrate is facilitated by these lesions.
    • Inner cytoplasmic membrane damage leads to inhibited respiration and enzyme leakage.

    Conclusions:

    • Complement and polymyxin act sequentially to disrupt gram-negative bacterial membranes.
    • This disruption facilitates the action of lysozyme and leads to cell death.
    • The findings provide insights into the bactericidal mechanisms of innate immunity and antibiotics.

    Related Experiment Videos