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Surface changes in temperature-sensitive Simian virus 40-transformed cells.

K D Noonan, H C Renger, C Basilico

    Proceedings of the National Academy of Sciences of the United States of America
    |February 1, 1973
    PubMed
    Summary
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    Temperature-sensitive simian virus 40-transformed 3T3 cells (tsSV3T3) show increased concanavalin A binding, a transformation marker, dependent on DNA synthesis. This binding change correlates with cell agglutination differences at varying temperatures, indicating cell surface alterations during viral transformation.

    Area of Science:

    • Cell Biology
    • Virology
    • Biochemistry

    Background:

    • Viral transformation alters cell surface properties.
    • Simian virus 40 (SV40) transformation of 3T3 cells leads to phenotypic changes.
    • Temperature-sensitive mutants (tsSV3T3) allow study of transformation-related events at different temperatures.

    Purpose of the Study:

    • To investigate kinetic changes in cell surface properties during SV40-induced transformation.
    • To correlate concanavalin A binding with the expression of the transformed phenotype.
    • To examine the role of DNA synthesis in these cell surface alterations.

    Main Methods:

    • Utilized a tritium-labeled concanavalin A binding assay.
    • Studied tsSV3T3 cells, which exhibit temperature-dependent transformation.

    Related Experiment Videos

  • Assessed cell agglutination with concanavalin A and wheat-germ agglutinin at different temperatures (32°C and 39°C).

    • Main Results:

    • Consistent differences in concanavalin A binding were observed between normal and virally transformed cells.
    • Increased concanavalin A binding paralleled the appearance of transformed cell characteristics.
    • This increased binding was dependent on cellular DNA synthesis.
    • tsSV3T3 cells showed significantly lower agglutination titers at 32°C compared to 39°C.

    Conclusions:

    • Cell surface changes, indicated by altered lectin binding and agglutination, are linked to viral transformation.
    • DNA synthesis is crucial for the expression of these transformation-associated cell surface alterations.
    • The temperature-sensitive nature of tsSV3T3 cells provides a model for studying the kinetics of these changes.