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Experience with an image-analyzing computer in virus plaque measurements.

W Scheirer

    Applied Microbiology
    |April 1, 1973
    PubMed
    Summary

    Image-analyzing computers offer fast and reliable plaque number determinations. This makes them highly valuable for large-scale screening programs utilizing plaque-reduction tests.

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    Area of Science:

    • Virology
    • Microbiology
    • Computer Science

    Background:

    • Plaque-reduction assays are standard for quantifying viral infectivity.
    • Accurate and rapid plaque enumeration is crucial for experimental throughput.

    Purpose of the Study:

    • To evaluate the utility of an image-analyzing computer system for plaque number determination.
    • To assess the speed and reliability of automated plaque counting.

    Main Methods:

    • Utilized an image-analyzing computer for plaque enumeration in plaque-reduction tests.
    • Compared computer-based determinations with manual counting methods.

    Main Results:

    • The image-analyzing computer provided extremely fast plaque-number determinations.
    • The system demonstrated high reliability in quantifying viral plaques.

    Conclusions:

    • Automated plaque counting using image-analyzing computers is highly effective.
    • This technology is particularly advantageous for large-scale screening programs and plaque-reduction assays.

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