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Related Experiment Videos

The second international standard for polymyxin B.

J W Lightbown, A H Thomas, B Grab

    Bulletin of the World Health Organization
    |January 1, 1973
    PubMed
    Summary

    A new international standard for Polymyxin B was established. Its potency was determined through a collaborative assay, defining the International Unit for this crucial antibiotic.

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    Area of Science:

    • Microbiology
    • Pharmacology
    • Biotechnology

    Background:

    • The first International Standard for Polymyxin B became unavailable, necessitating a replacement.
    • Polymyxin B is a critical antibiotic used to treat serious bacterial infections.

    Purpose of the Study:

    • To establish a Second International Standard for Polymyxin B.
    • To accurately determine the potency of the new standard through a collaborative assay.

    Main Methods:

    • A collaborative assay involving 5 laboratories across 4 countries was conducted.
    • Potency was estimated based on the results of this assay, with discrepant results being rejected.
    • Statistical analysis was used to determine the overall mean values.

    Main Results:

    • The agreed-upon potency for the Second International Standard for Polymyxin B was 8,403 IU/mg.
    • Potential difficulties in handling the first standard may have contributed to earlier result variations.
    • The World Health Organization (WHO) Expert Committee on Biological Standardization accepted this value.

    Conclusions:

    • The Second International Standard for Polymyxin B was successfully established and its potency defined.
    • The International Unit of polymyxin B was defined as the activity in 0.000119 mg of the second standard.
    • This standardization ensures consistent potency measurement for Polymyxin B globally.

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