Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A sensitive and accurate gel osmometer.

A G Ogston, B N Preston

    The Biochemical Journal
    |April 1, 1973
    PubMed
    Summary
    This summary is machine-generated.

    A novel polyacrylamide gel osmometer offers high sensitivity for measuring osmotic pressure and molecular weights. This method provides accurate results for various solutions, comparable to membrane osmometers.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The sedimentation constant of insulin.

    The Biochemical journal·2010
    Same author

    Spreading of boundaries in electrophoresis.

    Nature·2010
    Same author

    The definition and meaning of pH.

    Physiological reviews·2010
    Same author

    Interaction between polysaccharides and other substances. Non-equilibrium conditions.

    Upsala journal of medical sciences·2010
    Same author

    Partial characterization of matrix components interacting with cartilage proteoglycans.

    Archives of biochemistry and biophysics·2001
    Same author

    Dependence of salt concentration on glycosaminoglycan-lysozyme interactions in cartilage.

    Archives of biochemistry and biophysics·1997
    Same journal

    Mechanistic insights into acetylated histone recognition by the CECR2 bromodomain.

    The Biochemical journal·2026
    Same journal

    Nanobodies against Plasmodium adhesins that block receptor engagement and malaria parasite invasion.

    The Biochemical journal·2026
    Same journal

    Persistence without turnover: the RhoG G12E mutant highlights the role of nucleotide cycling in RhoG signaling.

    The Biochemical journal·2026
    Same journal

    Alternative Splicing of Rice Chloroplastic CuZn Superoxide Dismutase, OsCSD2: Impact on expression and protein characteristics.

    The Biochemical journal·2026
    Same journal

    Difference and similarity between the ubiquitous secretory pathway Ca2+-ATPases, SERCA2b, and SPCA1a.

    The Biochemical journal·2026
    Same journal

    A molecular perspective on dimethylarginine dimethylaminohydrolases structure and function.

    The Biochemical journal·2026
    See all related articles

    Area of Science:

    • Biochemistry
    • Physical Chemistry
    • Materials Science

    Background:

    • Osmotic pressure is a crucial colligative property for determining molecular weight.
    • Existing osmometry techniques have limitations in sensitivity or sample volume.
    • Polyacrylamide gels offer tunable properties for developing new measurement tools.

    Purpose of the Study:

    • To develop a novel osmometer utilizing polyacrylamide gel properties.
    • To assess the sensitivity and accuracy of the new gel-based osmometer.
    • To determine the molecular weight of various substances using this method.

    Main Methods:

    • A bilayer strip of cross-linked polyacrylamide gel on cellulose forms a circular loop.
    • Gel shrinkage due to osmotic pressure causes loop end separation, measured by microscopy.

    Related Experiment Videos

  • The osmometer was tested with solutions of human serum albumin, ovalbumin, cytochrome c, dextrans, polyvinyl alcohol, and polyethylene glycols.

    • Main Results:

    • The gel osmometer achieved a sensitivity of approximately 5 Pa, 30 times greater than Sephadex-bead osmometers.
    • Accurate molecular weight measurements (1-2% accuracy) were obtained for molecules as small as 1500 Da.
    • The method is effective for osmotic pressures up to 5 kPa, requiring only 0.5 ml of solution per measurement.

    Conclusions:

    • The polyacrylamide gel osmometer is a sensitive and accurate tool for osmotic pressure and molecular weight determination.
    • This method offers advantages in sensitivity and sample volume compared to traditional osmometers.
    • The technique is versatile, applicable to a wide range of biological and synthetic macromolecules.