Effect of phospholipase C on calcium release from epithelia treated with antidiuretic hormone

1. Addition of antidiuretic hormone (ADH) to the bladders of toads (Bufo marinus) preloaded with (45)Ca causes an increase in the rate of calcium efflux.2. Pretreatment of the serosal surfaces of bladders with phospholipase C prevents the action of ADH on calcium efflux.