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Related Experiment Videos

Estrogen-receptor interaction.

E V Jensen, E R DeSombre

    Science (New York, N.Y.)
    |October 12, 1973
    PubMed
    Summary
    This summary is machine-generated.

    Estradiol binding to uterine cells triggers receptor transformation, enhancing its nuclear binding and RNA synthesis capabilities. This hormone-induced conversion is crucial for estrogen

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    Estrogen receptor (ER) beta, a modulator of ERalpha in the uterus.

    Proceedings of the National Academy of Sciences of the United States of America·2000

    Area of Science:

    • Endocrinology
    • Molecular Biology
    • Cell Biology

    Background:

    • Estradiol interacts with uterine cells via extranuclear receptors.
    • This interaction initiates a temperature-dependent translocation to the nucleus.

    Purpose of the Study:

    • To investigate the process of "receptor transformation" in estradiol-uterine cell interactions.
    • To understand the functional significance of receptor transformation in estrogen action.

    Main Methods:

    • Studied the sedimentation rate changes of the estradiol-receptor complex.
    • Assessed the ability of the transformed complex to bind to uterine nuclei.
    • Evaluated the effect of the transformed complex on nuclear RNA synthesis.

    Main Results:

    Related Experiment Videos

    • Receptor transformation increased sedimentation rate from 3.8S to 5.2S.
    • Transformed complex gained the ability to bind uterine nuclei and enhance RNA synthesis.
    • Warming cytosol with estradiol induced transformation, mimicking nuclear-extracted complex properties.

    Conclusions:

    • Receptor transformation is a key step in estrogen action.
    • Estradiol induces the conversion of the receptor to a biochemically functional form.
    • This transformation is essential for modulating uterine cell nuclear activity.