Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spontaneous quantal transmitter release: a statistical analysis and some implications.

J I Hubbard, S F Jones

    The Journal of Physiology
    |July 1, 1973
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    TAK-228 (formerly MLN0128), an investigational dual TORC1/2 inhibitor plus paclitaxel, with/without trastuzumab, in patients with advanced solid malignancies.

    Cancer chemotherapy and pharmacology·2017
    Same author

    A phase IB trial of the oral MEK inhibitor trametinib (GSK1120212) in combination with everolimus in patients with advanced solid tumors.

    Annals of oncology : official journal of the European Society for Medical Oncology·2014
    Same author

    Phase I study of weekly paclitaxel in combination with pazopanib and lapatinib in advanced solid malignancies.

    British journal of cancer·2014
    Same author

    The mechanics of landing when stepping down in unilateral lower-limb amputees.

    Clinical biomechanics (Bristol, Avon)·2005
    Same author

    Postoperative NSAIDs and COX-2 inhibitors: cardiovascular risks and benefits.

    British journal of anaesthesia·2005
    Same author

    The gait initiation process in unilateral lower-limb amputees when stepping up and stepping down to a new level.

    Clinical biomechanics (Bristol, Avon)·2005

    Neurotransmitter release at the neuromuscular junction is not a simple Poisson process. Instead, it involves the coordinated, phased activity of numerous individual release sites, suggesting complex regulation of synaptic transmission.

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biophysics

    Background:

    • Miniature end-plate potentials (m.e.p.p.s) are fundamental units of neurotransmission.
    • Understanding the statistical properties of m.e.p.p. release is key to deciphering synaptic function.

    Purpose of the Study:

    • To investigate the statistical release patterns of neurotransmitters at the rat neuromuscular junction.
    • To determine the number and activity of neurotransmitter release sites.

    Main Methods:

    • Intra- and extracellular recording of m.e.p.p.s in rat phrenic nerve-diaphragm preparations.
    • Statistical analysis of m.e.p.p. intervals and variance-mean relationships.
    • Computer simulations to model release mechanisms.
    • Depolarization experiments to assess nerve terminal activity.

    Related Experiment Videos

    Main Results:

    • m.e.p.p. release deviates from a Poisson process, indicating coordinated release from multiple sites.
    • No evidence of 'drag' or 'clustering' in release probability.
    • Larger amplitude m.e.p.p.s likely result from supramodal vesicle volumes.
    • Depolarization increases site activity, not the number of sites.
    • Estimated at least 200 +/- 100 release sites per terminal.

    Conclusions:

    • Neurotransmitter release is regulated by the phased activity of numerous release sites.
    • The number of release sites correlates with ultrastructural observations.
    • Synaptic vesicle volume may influence m.e.p.p. amplitude.