Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

WHO collaborative studies on enterovirus reference antisera; fourth report.

J L Melnick, B Hampil

    Bulletin of the World Health Organization
    |January 1, 1973
    PubMed
    Summary

    This study evaluated the specificity of equine antisera for enteroviruses, finding generally good homologous antibody titers and limited heterotypic cross-reactivity. Results guide the use of these reference antisera for enterovirus detection.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cytomegalovirus genome and the immediate-early antigen in cells of different layers of human aorta.

    Virchows Archiv : an international journal of pathology·2000
    Same author

    Frequency of coexistence of cytomegalovirus and Chlamydia pneumoniae in atherosclerotic plaques.

    Central European journal of public health·2000
    Same author

    Cytomegalovirus infection and atherosclerosis.

    Central European journal of public health·1997
    Same author

    Cytomegalovirus infection, lipoprotein(a), and hypercoagulability: an atherogenic link?

    Arteriosclerosis, thrombosis, and vascular biology·1997
    Same author

    Cohort study of cytomegalovirus infection as a risk factor for carotid intimal-medial thickening, a measure of subclinical atherosclerosis.

    Circulation·1996
    Same author

    Current status of poliovirus infections.

    Clinical microbiology reviews·1996

    Area of Science:

    • Virology
    • Immunology

    Background:

    • Enteroviruses are significant human pathogens.
    • Standardized reference antisera are crucial for accurate enterovirus identification.
    • Previous studies established a program for evaluating equine antisera.

    Purpose of the Study:

    • To assess the specificity of equine antisera against prototype enteroviruses (Coxsackievirus and Echovirus types).
    • To evaluate homologous and heterotypic antibody responses in these antisera.
    • To provide guidance for the long-term use of enterovirus reference antisera.

    Main Methods:

    • Horses were inoculated with prototype enteroviruses (Coxsackievirus A2, 4, 8, 10-16, 18-21, 24; Echovirus 21, 27, 30, 31, 33).
    • Neutralizing antibody tests were performed against homologous viruses, regional homotypic strains, reoviruses, adenoviruses, and other enteroviruses.

    Related Experiment Videos

  • Geometric mean antibody titers were calculated for homologous and heterotypic reactions.

    • Main Results:

    • Homologous antibody titers were high, ranging from 3,000 to 10,000 for Echovirus antisera and 1,500 to 14,000 for most Coxsackievirus antisera.
    • Significant heterotypic antibody activity was detected in four antisera: Echovirus 31, Coxsackievirus A8, A13, and A15.
    • Homotypic test results with regional isolates were satisfactory.

    Conclusions:

    • The evaluated equine antisera demonstrate good specificity for their intended homologous enterovirus types.
    • Limited heterotypic antibody activity was observed, requiring consideration during diagnostic use.
    • These findings support the utility of the characterized antisera as reliable reference standards for enterovirus research and diagnostics.