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Viscosity of cellular protoplasm.

A D Keith, W Snipes

    Science (New York, N.Y.)
    |February 15, 1974
    PubMed
    Summary
    This summary is machine-generated.

    This study measured protoplasmic viscosity using a novel spin label technique. Results show viscosity varies significantly, highlighting the importance of membrane diffusion for cell function.

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    Area of Science:

    • Biophysics
    • Cell Biology
    • Membrane Biophysics

    Background:

    • Understanding cellular viscosity is crucial for cell function.
    • Previous methods for measuring intracellular viscosity have limitations.

    Purpose of the Study:

    • To investigate protoplasmic viscosity using a novel spin label approach.
    • To determine the variability of viscosity within cells and its implications.

    Main Methods:

    • Utilized a small spin label with high permeability and solubility.
    • Employed nickel chloride as an extracellular spin-subtracting agent to isolate intracellular signals.
    • Measured protoplasmic viscosity in various cell types.

    Main Results:

    • Protoplasmic viscosity was found to be highly variable among cells.

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  • In some cells, viscosity was significantly higher than water or phospholipids.
  • The findings suggest a dynamic intracellular environment.

    • Conclusions:

    • Lateral diffusion within biological membranes plays a critical role in cellular processes.
    • The developed spin label method is effective for probing intracellular environments.
    • Cellular viscosity is a dynamic property influencing cell function.