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Reassortment of simian virus 40 DNA during serial undiluted passage
Abstract:
Alterations occur in the supercoiled form of viral DNA after the serial undiluted passaging of simian virus (SV) 40. We have identified a portion of the viral genome which is amplified during this process. These SV40 DNA sequences represent about 30% of the viral genetic information and are present in a reiterated form in twisted circular molecules prepared from purified virions. In addition, reiterated and unique green monkey DNA sequences are incorporated into supercoiled viral DNA. The cellular DNA appears to be inserted at numerous locations in the DNA I molecules.
Insights
Serial passaging of simian virus 40 (SV40) causes viral DNA alterations. A significant portion of the SV40 genome is amplified and integrated with host DNA in supercoiled viral DNA molecules.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Serial undiluted passaging of simian virus 40 (SV40) leads to alterations in its supercoiled DNA.
- Understanding these alterations is crucial for comprehending viral genome dynamics and host-virus interactions.
Purpose of the Study:
- To identify specific viral genome regions amplified during serial passaging of SV40.
- To investigate the integration of host DNA into viral supercoiled DNA molecules.
Main Methods:
- Analysis of supercoiled DNA from purified SV40 virions after serial passaging.
- Identification and characterization of amplified viral and incorporated cellular DNA sequences.
Main Results:
- A specific portion, approximately 30% of the SV40 genome, is amplified during passaging.
- Amplified SV40 DNA sequences are found in a reiterated form within supercoiled viral DNA.
- Reiterated and unique green monkey DNA sequences are incorporated into the supercoiled viral DNA, with cellular DNA inserted at multiple sites.
Conclusions:
- Serial passaging induces significant genomic changes in SV40, including amplification of viral DNA segments.
- SV40 DNA can incorporate host cellular DNA, suggesting a mechanism for genetic exchange between virus and host.