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Related Experiment Videos

Elemental composition of platelets. Part I. Sampling and sample preparation of platelets for trace-element analysis.

G V Iyengar, H Borberg, K Kasperek

    Clinical Chemistry
    |May 1, 1979
    PubMed
    Summary

    This study presents a refined method for collecting pure platelet samples for trace-element analysis. The optimized technique ensures sample integrity and minimizes contamination, crucial for accurate elemental quantification in platelets.

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    Area of Science:

    • Biochemistry
    • Hematology
    • Analytical Chemistry

    Background:

    • Platelet sampling for trace-element analysis faces challenges including purity, viability, trapped plasma, and contamination.
    • Accurate elemental analysis requires overcoming these sampling hurdles to ensure reliable results.

    Purpose of the Study:

    • To develop and validate a robust method for isolating pure, viable platelets for trace-element analysis.
    • To establish optimal conditions for differential centrifugation and contamination control during platelet collection.

    Main Methods:

    • Utilized a blood-cell separator for initial platelet isolation, followed by differential centrifugation in natural plasma.
    • Employed pyrimidopyrimidine RA233 as a viability stabilizer and 131I-labeled human serum albumin to quantify trapped plasma.

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  • Controlled contamination using five-times-distilled water and analyzed three blood fractions; elemental analysis performed via neutron activation analysis.

    • Main Results:

    • Optimized differential centrifugation at 400 x g for 8 min yielded high platelet purity (erythrocytes/platelets < 5 mg/g, leukocytes/platelets < 20 mg/g).
    • Trapped plasma was quantified at approximately 0.40 g/g in wet platelet samples.
    • Neutron activation analysis confirmed no significant contamination for a range of elements (Ag, Cd, Co, Cr, Cs, Cu, Fe, Mo, Rb, Sb, Se, Zn).

    Conclusions:

    • The developed method effectively isolates pure and viable platelets suitable for trace-element analysis.
    • The protocol minimizes contamination and accurately corrects for trapped plasma, enhancing the reliability of elemental quantification in platelets.