Related Experiment Video
Updated: Aug 6, 2026

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
Published on: October 28, 2014
Intracellular cyclic AMP concentration responds specifically to growth regulation by serum
Abstract:
Intracellular concentrations of cyclic AMP increase when cells are deprived of serum. Studies with the mouse fibroblast line 3T3, an SV40-transformed subline of 3T3, and six different revertant lines derived from this clone show that a marked increase in cyclic AMP occurs only when the serum concentration is reduced below the minimum necessary for growth of a given line. Conversely, density-dependent inhibition of growth is not accompanied by an increase in cyclic AMP concentration in any line.
More Related Videos
08:22Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and λ) Hyperspectral FRET Imaging and Analysis
Published on: October 27, 2020
06:03Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In Situ
Published on: March 22, 2024
Related Concept Videos
Intracellular Signaling Cascades
Positive Regulator Molecules
Amplifying Signals via Second Messengers
cAMP-dependent Protein Kinase Pathways
Feedback Regulation of Calcium Concentration
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...