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Cytomegalovirus interference in vitro.

L A Glasgow

    Infection and Immunity
    |April 1, 1974
    PubMed
    Summary
    This summary is machine-generated.

    Cytomegalovirus (CMV) induces viral interference in human embryo fibroblast (HEF) cultures. This interference, mediated by a soluble factor, confers resistance to various viruses, mimicking interferon effects.

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    Area of Science:

    • Virology
    • Cell Biology
    • Immunology

    Background:

    • Cytomegalovirus (CMV) is a common human virus.
    • Viral interference describes a phenomenon where one virus inhibits the replication of another.
    • Human embryo fibroblast (HEF) cultures are a standard model for studying viral infections.

    Purpose of the Study:

    • To investigate the mechanism by which Cytomegalovirus (CMV) induces viral interference in human embryo fibroblast (HEF) cultures.
    • To characterize the nature of the soluble mediator responsible for this interference.
    • To compare the CMV-induced interference with the effects of human interferon.

    Main Methods:

    • Infection of human embryo fibroblast (HEF) cultures with Cytomegalovirus (CMV).
    • Characterization of the interference phenomenon, including latent period, specificity, and dependence on infectious virus.

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  • Analysis of the soluble mediator produced during CMV-cell interaction, including its resistance to anti-CMV antibodies and its effects on virus adsorption.
  • Comparison with the effects of exogenous human interferon exposure.

    • Main Results:

    • CMV infection established a state of viral interference in HEF cultures after a latent period of 48-96 hours.
    • This interference was effective against a range of heterologous viruses but was overcome by high multiplicities of infection and did not affect virus adsorption.
    • A soluble mediator, distinct from anti-CMV antibodies, was responsible for the interference and shared properties with interferon, though no detectable interferon was present.

    Conclusions:

    • Cytomegalovirus (CMV) induces a unique state of viral interference in human embryo fibroblast (HEF) cultures.
    • The interference is mediated by a soluble, interferon-like factor produced by CMV-infected cells.
    • This CMV-induced mediator confers resistance to superinfecting viruses, highlighting a novel host-virus interaction mechanism.