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Harvey sarcoma virus: a second murine type C sarcoma virus with rat genetic information
Journal of Virology
|June 1, 1974
Summary
The Harvey sarcoma virus contains genetic sequences from both mouse and rat origins. These findings suggest a recombination event between mouse retroviruses and rat cell components led to its formation.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Murine sarcoma viruses (MSVs) are retroviruses known to cause tumors in mice.
- The Harvey strain of MSV (HaMSV) has unique biological properties suggesting a complex genetic origin.
Purpose of the Study:
- To analyze the nucleic acid sequences within the Harvey strain of murine sarcoma virus.
- To determine the origin and relationship of these sequences to known viral and cellular genomes.
Main Methods:
- Utilized RNA-DNA hybridization techniques with radiolabeled RNA probes.
- Employed [(3)H]DNA and [(3)H]RNA probes for sequence analysis.
Main Results:
- HaMSV possesses at least two distinct sets of nucleic acid sequences.
- One sequence set is shared with Moloney strain of mouse type-C virus.
- Another set is related to rat type-C viruses and found in Kirsten MSV, suggesting a rat origin.
Conclusions:
- HaMSV likely arose from recombination between mouse type-C viruses and rat cellular sequences.
- The transforming ability of HaMSV may originate from rat type-C viruses or the rat cellular genome.