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Related Experiment Videos

Rhabdovirus replication in enucleated host cells.

T J Wiktor, H Koprowski

    Journal of Virology
    |August 1, 1974
    PubMed
    Summary
    This summary is machine-generated.

    Rabies virus infection in enucleated monkey cells produced viral RNA and antigens but no infectious virus. However, these cells still produced infectious vesicular stomatitis virus, suggesting distinct viral replication requirements.

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    Area of Science:

    • Virology
    • Cell Biology
    • Molecular Biology

    Background:

    • Enucleated cells lack a nucleus, offering a model to study cytoplasmic viral replication.
    • Rabies virus is an RNA virus that replicates in the cytoplasm.
    • Vesicular stomatitis virus is another RNA virus with different replication strategies.

    Purpose of the Study:

    • To investigate the role of the nucleus in rabies virus replication.
    • To compare the replication of rabies virus and vesicular stomatitis virus in enucleated cells.
    • To assess the impact of mitochondrial function on viral replication in enucleated cells.

    Main Methods:

    • Infection of enucleated TC-7 monkey cells with rabies virus and vesicular stomatitis virus.
    • Analysis of virus-directed RNA synthesis and antigen production.

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  • Measurement of infectious virus yield.
  • Inhibition of mitochondrial functions using ethidium bromide.

    • Main Results:

    • Enucleated cells infected with rabies virus synthesized viral RNA and antigens but did not produce infectious virus.
    • Infectious vesicular stomatitis virus yield was comparable in enucleated and intact cells.
    • Ethidium bromide treatment did not affect rabies antigen production or vesicular stomatitis virus yield in enucleated cells.

    Conclusions:

    • The nucleus is essential for the production of infectious rabies virus.
    • Cytoplasmic replication is sufficient for vesicular stomatitis virus production in enucleated cells.
    • Mitochondrial function is not critical for the observed replication of these viruses in enucleated cells.