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Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
Published on: August 24, 2015
Rapid typing of herpes simplex virus strains using the indirect immunoperoxidase method
Applied Microbiology
|October 1, 1974
Summary
The indirect immunoperoxidase technique offers a simpler and faster method for typing herpes simplex virus strains compared to kinetic neutralization. Both methods showed complete agreement in identifying virus types.
Area of Science:
- Virology
- Immunology
Background:
- Herpes simplex virus (HSV) typing is crucial for clinical and epidemiological studies.
- Accurate and efficient methods for HSV strain identification are needed.
Purpose of the Study:
- To compare the indirect immunoperoxidase technique with kinetic neutralization for HSV typing.
- To evaluate the simplicity, interpretability, and speed of the indirect immunoperoxidase assay.
Main Methods:
- Utilized type-specific antisera for the indirect immunoperoxidase technique.
- Employed kinetic neutralization assays for comparison.
- Applied both methods to type herpes simplex virus strains.
Main Results:
- Complete agreement was observed between the indirect immunoperoxidase technique and kinetic neutralization.
- The indirect immunoperoxidase assay demonstrated superior simplicity and ease of interpretation.
- The indirect immunoperoxidase method provided a rapid approach for routine HSV antigenic typing.
Conclusions:
- The indirect immunoperoxidase technique is a reliable and efficient alternative to kinetic neutralization for HSV typing.
- This method facilitates faster and simpler routine identification of herpes simplex virus isolates.
