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Related Experiment Videos

Human T lymphocyte "E" rosette function. I. A process modulated by intracellular cyclic AMP.

F V Chisari, T S Edgington

    The Journal of Experimental Medicine
    |October 1, 1974
    PubMed
    Summary

    Elevating intracellular cyclic AMP levels inhibits normal human T lymphocyte rosette formation with sheep red blood cells. This finding suggests cyclic AMP plays a key role in regulating T lymphocyte E rosette function.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • Normal human T lymphocytes form rosettes with sheep red blood cells, a function potentially modulated by intracellular signaling.
    • Cyclic AMP (cAMP) is a crucial second messenger involved in various cellular processes.

    Purpose of the Study:

    • To investigate the effect of elevated intracellular cyclic AMP levels on the E rosette function of normal human T lymphocytes.

    Main Methods:

    • T lymphocytes were treated with agents known to increase intracellular cyclic AMP, such as isoproterenol, aminophylline, and cholera toxin.
    • The inhibitory effects of these agents and dibutyryl cyclic AMP on E rosette formation were observed.
    • The role of phosphodiesterase inhibition in potentiating these effects was also examined.

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    Main Results:

    • Drugs and agents that increase intracellular cyclic AMP levels were found to inhibit T lymphocyte E rosette formation.
    • Rapid increases in cyclic AMP (e.g., isoproterenol, aminophylline) led to early inhibition, while cholera toxin caused a delayed inhibition.
    • Dibutyryl cyclic AMP also inhibited rosette function, and its effects were enhanced by phosphodiesterase inhibitors.

    Conclusions:

    • Elevation of intracellular cyclic AMP levels significantly diminishes the E rosette function of human T lymphocytes.
    • These findings provide strong evidence for the role of cyclic AMP in regulating T lymphocyte-sheep red blood cell interactions.