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Related Experiment Videos

The N protein of bacteriophage lambda, defined by its DNA sequence, is highly basic.

N C Franklin, G N Bennett

    Gene
    |December 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

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    Researchers determined the nucleotide sequence of bacteriophage lambda's N gene, revealing its unique reading frame and translation start/stop points. This analysis defines the precise coding sequence for the bacteriophage lambda N protein.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Virology

    Background:

    • The pL-N-tL1 region of bacteriophage lambda contains the essential N gene.
    • Understanding gene expression requires precise determination of nucleotide sequences and reading frames.

    Purpose of the Study:

    • To determine the nucleotide sequence of the pL-N-tL1 region of bacteriophage lambda.
    • To define the unique reading frame and translation initiation/termination sites for the bacteriophage lambda N gene.

    Main Methods:

    • Nucleotide sequencing of restriction fragments and cloned DNA from the bacteriophage lambda pL-N-tL1 region.
    • Analysis of natural and mutation-induced nonsense codons to identify the functional reading frame.

    Main Results:

    Related Experiment Videos

  • A unique reading frame for the N gene was identified, characterized by the absence of natural stop codons and the presence of seven mutation-generated nonsense codons.
  • Translation initiation occurs at two alternative ATG codons, with the second likely serving as the in vivo start site.
  • Translation termination is signaled by a single TAG codon, resulting in a protein of 133 or, more likely, 107 amino acids, rich in lysine, arginine, and proline.

    • Conclusions:

    • The study precisely defines the genetic code for the bacteriophage lambda N protein.
    • This detailed sequence information is crucial for understanding N protein function and regulation in bacteriophage lambda.