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Related Experiment Videos

Structural characteristics of interferons from mouse Ehrlich ascites tumor cells.

B Cabrer, H Taira, R J Broeze

    The Journal of Biological Chemistry
    |May 25, 1979
    PubMed
    Summary

    Researchers isolated three interferon sizes from mouse ascites tumor cells. Differences in size are likely due to variations in glycosylation, impacting interferon function.

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    Super high resolution for single molecule-sequence-based typing of classical HLA loci at the 8-digit level using next generation sequencers.

    Tissue antigens·2012

    Area of Science:

    • Immunology
    • Virology
    • Biochemistry

    Background:

    • Interferons (IFNs) are crucial antiviral proteins.
    • Mouse Ehrlich ascites tumor cells infected with Newcastle disease virus (NDV) are a source of IFNs.
    • Understanding IFN heterogeneity is important for their therapeutic applications.

    Purpose of the Study:

    • To improve the isolation procedure for mouse interferons.
    • To characterize the different size classes of interferons produced.
    • To investigate the structural basis for size heterogeneity in interferons.

    Main Methods:

    • Isolation of interferons from NDV-infected mouse Ehrlich ascites tumor cells.
    • Determination of interferon size classes using molecular weight markers.
    • Analysis of interferon structure using tryptic peptide mapping and amino acid composition.

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  • NH2-terminal amino acid sequencing and carboxypeptidase A treatment.

    • Main Results:

    • An improved procedure yielded interferons in three size classes: 33,000, 26,000, and 20,000 daltons.
    • Specific activities ranged from 2 to 3 x 10(9) units/mg protein, with a yield of 11-20%.
    • Tryptic peptide maps indicated similarity between the two larger species, but differences in the smallest species.
    • Amino acid composition, NH2-terminal sequences, and carboxypeptidase A digestion patterns were nearly identical across all three size classes.

    Conclusions:

    • The improved isolation procedure effectively produced multiple interferon size classes.
    • Structural analysis suggests that size heterogeneity is primarily due to differences in glycosylation.
    • These findings contribute to understanding interferon structure-function relationships and potential therapeutic modifications.