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Cell volume decrease during Friend leukemia cell differentiation.

R M Zucker, N C Wu, A Mitrani

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |January 1, 1979
    PubMed
    Summary
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    Dimethyl sulfoxide and butyric acid induce Friend leukemia cell differentiation, with cell volume changes rapidly monitoring early events. Kinetics vary by agent and concentration, showing a transient G1 block and volume decrease during differentiation.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Hematology

    Background:

    • Friend leukemia cells are a model system for studying cellular differentiation.
    • Understanding the kinetics of differentiation is crucial for developing therapeutic strategies.

    Purpose of the Study:

    • To investigate the kinetics of Friend leukemia cell differentiation induced by dimethyl sulfoxide (DMSO) and butyric acid.
    • To determine the role of cell volume changes as an early indicator of differentiation.

    Main Methods:

    • Friend leukemia cells (GM86, clone 745) were induced to differentiate using DMSO or butyric acid.
    • Kinetics were measured by cell growth, cell volume distributions, and [3H]thymidine incorporation.
    • Cell cycle analysis was performed to identify changes in cell cycle distribution.

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    Main Results:

    • Differentiation induction was agent-sensitive and concentration-dependent.
    • DMSO induction showed earlier volume distribution changes than butyric acid.
    • A transient G1 block and cell volume decrease correlated with differentiation initiation, followed by an increase in large cells and labeling indices.

    Conclusions:

    • Cell volume changes serve as a rapid monitor for early differentiation events in Friend leukemia cells.
    • Differentiation involves a transient G1 block and cell volume decrease related to cell cycle redistribution.
    • DMSO and butyric acid exhibit distinct concentration-dependent effects on differentiation kinetics.