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Related Experiment Videos

Cellular autofluorescence--is it due to flavins?

R C Benson, R A Meyer, M E Zaruba

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |January 1, 1979
    PubMed
    Summary

    Cellular autofluorescence in the 500-600 nm range creates background noise for sensitive fluorescence measurements. This study demonstrates that endogenous flavoproteins are the likely source of this autofluorescence in many cell types.

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    Area of Science:

    • Cell Biology
    • Biochemistry
    • Spectroscopy

    Background:

    • Cellular autofluorescence, particularly in the 500-600 nm spectral region, poses a significant challenge for detecting weakly fluorescent probes.
    • This background signal can obscure or interfere with quantitative fluorescence measurements in various cell types.

    Purpose of the Study:

    • To investigate the source of cellular autofluorescence in the 500-600 nm spectral region.
    • To identify the endogenous molecules responsible for this autofluorescence in diverse cell types.

    Main Methods:

    • Measurement of fluorescence intensity and spectra from various cell types.
    • Analysis of neuronal homogenates to assess autofluorescence characteristics.

    Main Results:

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    • Autofluorescence was observed in the 500-600 nm range across multiple cell types.
    • Spectral analysis indicated a consistent pattern suggestive of a specific endogenous source.
    • Evidence points towards flavoproteins as the primary contributors to this autofluorescence.

    Conclusions:

    • Endogenous flavoproteins are identified as the most probable cause of cellular autofluorescence in the 500-600 nm range.
    • Understanding this autofluorescence is crucial for improving the accuracy of fluorescence-based assays and probe measurements.