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Related Experiment Videos

Sensitive GLC assay for pemoline in biological fluids using nitrogen-specific detection.

D J Hoffman

    Journal of Pharmaceutical Sciences
    |April 1, 1979
    PubMed
    Summary

    This study introduces a method for detecting pemoline in biological samples. Extractive alkylation and gas-liquid chromatography (GLC) allow sensitive quantification of pemoline in serum and urine.

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    Area of Science:

    • Analytical Chemistry
    • Pharmacology

    Background:

    • Pemoline is a stimulant drug with therapeutic uses.
    • Accurate quantification of pemoline in biological matrices is crucial for pharmacokinetic and toxicological studies.

    Purpose of the Study:

    • To develop and validate a sensitive method for determining intact pemoline in human serum and urine.
    • To establish a reliable analytical technique for pemoline quantification.

    Main Methods:

    • Extractive alkylation of pemoline using tetrapentylammonium hydroxide under alkaline conditions.
    • Formation of the N,N-dimethylpemoline derivative via methyl iodide treatment.
    • Gas-liquid chromatography (GLC) analysis utilizing a 5% FFAP column and nitrogen-specific detection.

    Main Results:

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    • The method achieved a sensitivity of 0.05 microgram/ml for both serum and urine samples.
    • Linear calibration curves were observed up to 4 microgram/ml in serum and 15 microgram/ml in urine.
    • Excellent precision was demonstrated with a pooled relative standard deviation of +/- 7.5% for serum samples.

    Conclusions:

    • Extractive alkylation followed by GLC provides a sensitive and precise method for pemoline determination.
    • This validated method is suitable for analyzing pemoline concentrations in clinical and forensic toxicology settings.