Relation of potassium transport to oxidative metabolism in isolated brain capillaries

1. The uptake of K by a capillary suspension isolated from rat brain was studied with the radioactive analogue (86)Rb.2. Rb uptake was dependent upon the presence of oxygen and could be markedly inhibited with ouabain.3. The ouabain sensitive Rb uptake was measured at varying external concentrations of K. Uptake of K (as (86)Rb) was half-maximal when the K concentration was 3.0 mM. This in vitro affinity of the transport carrier for K is similar to that found in previous in vivo studies of K efflux from brain to blood.4. I propose that the ouabain sensitive K pump is located on the antiluminal plasma membrane of brain capillary endothelial cells and that this pump contributes to the maintenance of a constant concentration (i.e. 3 mM) of K in brain interstitial fluid.5. Glucose and palmitate were tested as possible energy substrates for the support of active Rb uptake by isolated brain capillaries. The rate of Rb uptake increased 40% when 0.25 mM-palmitate was added to a capillary suspension containing 5 mM-glucose. This stimulation of Rb uptake could be blocked by 1 mM-4-pentenoic acid, an inhibitor of fatty acid oxidation. In contrast, the fraction of Rb uptake supported by glucose was not altered by 4-pentenoic acid.6. The rates of [U-(14)C]glucose and [U-(14)C]palmitate oxidation to CO(2) were measured in isolated brain capillaries and compared to their oxidation by brain slices and synaptosomes. Palmitate was the source of 28% of the (14)CO(2) produced by the capillaries but only 0.5% of the (14)CO(2) produced by the brain slices and synaptosomes.7. It is concluded that brain capillaries are similar to renal tubules in their polar distribution of ouabain sensitive K transport carriers, dependence on oxidative metabolism for active ion transport, and use of fatty acids as energy substrates. These features may underlie the vulnerability of brain capillaries in several metabolic diseases that cause brain oedema.