Related Experiment Videos
The laboratory transmission of Coxsackie A6 virus by mosquitoes
Abstract:
Coxsackie A 6 virus, strain V 29, originally isolated from Aedes polynesiensis in Fiji, was found to survive in A. australis for 5 days after intrathoracic injection and for 6 days after feeding on viraemic mice, and in Culiseta tonnoiri for 8 days after feeding.The virus level in both fed and injected mosquitoes fell steadily after infection and never exceeded the original level.No virus transmission was obtained in 46 successful second feedings on days 10-14 with A. australis, but three transmissions were obtained with C. tonnoiri, two on day 10 and one on day 13, from 12 successful second feedings.
Insights
Coxsackie A 6 virus survived in mosquitoes for up to 8 days. While Aedes australis did not transmit the virus, Culiseta tonnoiri showed limited transmission potential.
Area of Science:
- Virology
- Medical Entomology
- Arbovirology
Background:
- Coxsackie A 6 virus is an enterovirus.
- Mosquitoes are vectors for various viral pathogens.
Purpose of the Study:
- To investigate the survival and transmission capabilities of Coxsackie A 6 virus in Aedes australis and Culiseta tonnoiri mosquitoes.
Main Methods:
- Mosquitoes were infected with Coxsackie A 6 virus via intrathoracic injection or feeding on viraemic mice.
- Virus survival was assessed by titration at various time points.
- Transmission efficiency was evaluated through second feeding attempts on susceptible hosts.
Main Results:
- Coxsackie A 6 virus survived in Aedes australis for up to 6 days and in Culiseta tonnoiri for up to 8 days.
- Virus titers declined post-infection in both mosquito species.
- No transmission was observed with Aedes australis, but limited transmission occurred with Culiseta tonnoiri.
Conclusions:
- Culiseta tonnoiri exhibits a greater potential for transmitting Coxsackie A 6 virus compared to Aedes australis.
- Further research is needed to fully understand the vector competence of different mosquito species for Coxsackie A 6 virus.