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Updated: Aug 16, 2026
![Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F55858.jpg&w=3840&q=50)
Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
Published on: December 4, 2017
Studies on energy-linked reactions. Energy-linked transhydrogenase reaction in Escherichia coli
Abstract:
1. Small particles from Escherichia coli catalyse an ATP-dependent reduction of NADP(+) by NADH. 2. The reaction was stimulated by Mg(2+) and only ATP and ITP could serve as energy donors. 3. Studies of the stoicheiometry of the reaction indicate that 1-2mol of ATP are utilized/mol of NADH produced. 4. The reaction is inhibited by uncoupling agents such as 2,4-dinitrophenol (200mum) and tetrachlorotrifluorobenzimidazole (2mum) but oligomycin does not inhibit. 5. The reaction is inhibited by relatively high concentrations (200mum) of piericidin A.
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