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[Complementary interaction of multialkylated polyribonucleotides].

G L Dianov, T S Bondar', R I Salganik

    Molekuliarnaia Biologiia
    |March 1, 1979
    PubMed
    Summary

    Modification of polyinosinic acid (poly(I)) with an alkylating agent impacts its binding with polycytidylic acid (poly(C)). Even minor modifications affect complex formation and stability, suggesting potential for targeted genomic interactions.

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    Regulation of DNA repair by ubiquitylation.

    Biochemistry. Biokhimiia·2011

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Nucleic Acid Chemistry

    Context:

    • Polyinosinic acid (poly(I)) is a synthetic double-stranded RNA molecule.
    • Polycytidylic acid (poly(C)) is its complementary polynucleotide.
    • Chemical modification of nucleic acids can alter their biological properties.

    Purpose:

    • To investigate the effect of alkylating agent modification on poly(I):poly(C) complex formation.
    • To determine the threshold of modification that disrupts complementary interactions.
    • To assess the impact of modification on the stability and structure of the poly(I):poly(C) complex.

    Summary:

    • Modification of up to 10% of inosine residues in poly(I) allows retention of complementary complex formation with poly(C).
    • Complex formation capacity is lost when 11-13% of inosine residues are modified.
    • Modifications exceeding 3.5% render the poly(I):poly(C) complex sensitive to RNAse T1 and decrease its melting temperature (Tm), indicating the formation of single-stranded regions.

    Impact:

    • Modified poly(I) retains some complementary binding ability, suggesting potential applications.
    • The study highlights the sensitivity of nucleic acid complex formation to chemical modifications.
    • Modified polynucleotides with alkylating groups may be useful for targeted interactions with genomic regions.

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