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Pneumococci producing beta hemolysis on agar.

V Lorian, B Popoola

    Applied Microbiology
    |July 1, 1972
    PubMed
    Summary
    This summary is machine-generated.

    Certain pneumococci strains produce a novel beta hemolysin active against horse and sheep red blood cells. This substance requires specific anaerobic and aerobic conditions for activation and appears distinct from previously identified pneumococcal hemolysins.

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    Area of Science:

    • Microbiology
    • Bacteriology
    • Hemolysis

    Background:

    • Streptococcus pneumoniae (pneumococci) are significant human pathogens.
    • Hemolysins are virulence factors produced by various bacteria, including pneumococci.
    • Understanding bacterial hemolysins is crucial for elucidating pathogenicity and developing diagnostics.

    Purpose of the Study:

    • To investigate the hemolytic activity of pneumococcal strains on different blood-agar media.
    • To characterize the conditions required for the production and activation of a novel hemolytic substance.
    • To determine if serological type correlates with hemolytic activity.

    Main Methods:

    • Culturing fifty-six pneumococcal strains on blood-agar plates.
    • Incubating cultures anaerobically for 24 hours.

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  • Exposing cultures to air at low temperatures (6-20°C) for 48 hours to assess activation.
  • Observing and quantifying beta hemolysis against horse, sheep, human, and rabbit red blood cells.

    • Main Results:

    • Twenty-two (39%) of the pneumococcal strains exhibited beta hemolysis on horse red cells.
    • Six (11%) of the strains showed beta hemolysis on sheep red cells.
    • No lysis of human or rabbit red cells was observed.
    • The hemolytic substance appeared after anaerobic incubation and required subsequent aerobic activation at low temperatures.
    • No correlation was found between serological type and beta hemolysis production.

    Conclusions:

    • A novel beta hemolysin produced by certain pneumococcal strains has been identified.
    • This hemolysin specifically targets horse and sheep red blood cells.
    • The activation of this substance is dependent on specific anaerobic and aerobic environmental conditions.
    • The identified hemolysin appears to be distinct from previously described pneumococcal hemolysins.