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Updated: Oct 2, 2026
An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood
Published on: June 23, 2017
[Repeated sampling of rat polymorphonuclear leucocytes from the pleural cavity. Application for the study of
Abstract:
A method is described for allowing the sampling of a homogeneous population of polymorphonuclear cells (PMN) from the rat pleural cavity a well as a technique involving the use of a phase-contrast microscope and video-recording system to study the necrotaxis of rat PMN towards a single erythrocyte lysed by ruby-laser irradiation. This allows both the speed of the leucocytes and the degree of their chemotactic response to be determined. The advantages and disadvantages of the experimental technique are discussed along with its application to the study of in vitro and in vivo actions of various drugs on rat PMN chemotaxis.
Insights
Researchers developed a new method to isolate polymorphonuclear cells (PMN) from rat pleural cavities. This technique enables precise study of PMN necrotaxis and drug effects on their chemotaxis.
Area of Science:
- Immunology
- Cell Biology
- Microscopy Techniques
