Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tritiated bile acids: problems and recommendations.

D K Panveliwalla, D Pertsemlidis, E H Ahrens

    Journal of Lipid Research
    |September 1, 1974
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The Lipid Disturbance in Biliary Obstruction and its Relationship to the Genesis of Arteriosclerosis.

    Bulletin of the New York Academy of Medicine·2009
    Same author

    Development of a novel self-medicating applicator for control of internal and external parasites of wild and domestic animals.

    The Onderstepoort journal of veterinary research·2004
    Same author

    Diagnostic and interventional laparoscopy and intraoperative ultrasonography in the management of pancreatic disease.

    The Surgical clinics of North America·2001
    Same author

    Pheochromocytoma: inherited associations, bilaterality, and cortex preservation.

    Surgery·2000
    Same author

    Control of Boophilus annulatus (Acari: Ixodidae) on cattle using injectable microspheres containing ivermectin.

    Journal of economic entomology·1999
    Same author

    Efficacy of amitraz (Taktic 12.5% EC) as a dip for the control of Boophilus microplus (Canestrini) (Acari: Ixodidae) on cattle.

    Preventive veterinary medicine·1999

    Kinetic studies of bile acids revealed errors from chemical impurities and tritium loss. Precautions are essential when using tritiated bile acids for pool size and turnover rate research.

    Area of Science:

    • Biochemistry
    • Pharmacokinetics
    • Metabolic Research

    Background:

    • Bile acids, including cholic and chenodeoxycholic acids, are crucial for digestion and metabolism.
    • Accurate kinetic studies are vital for understanding bile acid metabolism and related diseases.

    Purpose of the Study:

    • To investigate the kinetics of cholic and chenodeoxycholic acids in human subjects.
    • To identify potential sources of error in kinetic studies using radiolabeled bile acids.

    Main Methods:

    • Simultaneous intravenous administration of paired (3)H- and (14)C-labeled cholic and chenodeoxycholic acids.
    • Kinetic analysis of radiolabeled bile acid distribution and elimination in three patients.

    Main Results:

    Related Experiment Videos

  • Identified chemical impurity of radiolabeled compounds as a significant error source.
  • Demonstrated loss of tritium by biological exchange, affecting kinetic data accuracy.
  • Highlighted the impact of these errors on pool size and turnover rate determinations.

    • Conclusions:

    • Standardized precautions are necessary for reliable use of tritiated bile acids in kinetic studies.
    • Addressing chemical purity and tritium exchange is critical for accurate bile acid metabolism research.
    • Findings provide guidance for improving the methodology of bile acid kinetic studies.