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Updated: Aug 18, 2026

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
Published on: June 26, 2013
Fluorescent retrograde double labeling: axonal branching in the ascending raphe and nigral projections
Abstract:
Red fluorescent Evans blue and blue fluorescent DAPI-primuline were injected into the anterior-medial and lateral-caudal forebrains, respectively, of the same rats. Separate clusters of cells labeled by retrograde transport were observed in the substantia nigra, while in the dorsal raphe many cells were double-labeled. Thus, single raphe cells send divergent axon collaterals to widespread forebrain areas.
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