Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Urea utilization by Leptospira.

S Kadis, W L Pugh

    Infection and Immunity
    |October 1, 1974
    PubMed
    Summary
    This summary is machine-generated.

    Pathogenic Leptospira strains utilize urea as a nitrogen source, with urease activity correlating to growth. This suggests a role for leptospiral urease in kidney localization and pathology.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Synchronous occurrence of a primary colon adenocarcinoma and a gastric stromal tumor. A case report.

    Minerva gastroenterologica e dietologica·2008
    Same author

    3-hour postprandial pH monitoring study correlation with endoscopy in gastroesophageal reflux disease.

    Journal of the College of Physicians and Surgeons--Pakistan : JCPSP·2007
    Same author

    Cameron lesion and its laparoscopic management.

    Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology·2005
    Same author

    Glucagon-like peptide-1 stimulates insulin secretion by a Ca2+-independent mechanism in Zucker diabetic fatty rat islets of Langerhans.

    Metabolism: clinical and experimental·2001
    Same author

    Prevention of diabetes does not completely prevent insulin secretory defects in the ZDF rat.

    The American journal of physiology·1995
    Same author

    Alterations in pulsatile insulin secretion in the Zucker diabetic fatty rat.

    The American journal of physiology·1994
    Same journal

    <i>Porphyromonas gingivalis</i> sphingolipids affect early responses of THP-1 macrophages to outer membrane vesicles.

    Infection and immunity·2026
    Same journal

    A genome-wide CRISPR screen defines host determinants of early <i>Brucella</i> infection in human macrophage-like cells.

    Infection and immunity·2026
    Same journal

    Antiphospholipid antibodies in acute and post-treatment Lyme disease.

    Infection and immunity·2026
    Same journal

    The cholesterol-dependent cytolysin promotes <i>Streptococcus</i> systemic spread and induces arachidonic acid accumulation-mediated lethality in a murine intraperitoneal infection model.

    Infection and immunity·2026
    Same journal

    Phenotypic and genotypic analysis of <i>Candida albicans</i> vaginal isolates reveals that <i>ECE1</i> expression underpins pathogenicity.

    Infection and immunity·2026
    Same journal

    <i>Staphylococcus aureus</i> pore-forming toxins differentially shape disease severity in experimental endophthalmitis.

    Infection and immunity·2026
    See all related articles

    Area of Science:

    • Microbiology
    • Bacteriology
    • Pathogenesis

    Background:

    • Leptospira are pathogenic bacteria causing leptospirosis.
    • Urease is an enzyme that hydrolyzes urea.
    • The role of urease in Leptospira pathogenesis is not fully understood.

    Purpose of the Study:

    • To investigate the ability of different Leptospira serotypes to utilize urea.
    • To determine the urease activity of pathogenic and saprophytic Leptospira strains.
    • To explore the potential role of leptospiral urease in kidney localization and pathology.

    Main Methods:

    • Cultivation of Leptospira strains on urea-containing media.
    • Assaying urease activity in intact and disrupted cells.
    • Analyzing growth patterns in the presence of ammonium chloride and urea.

    Related Experiment Videos

    Main Results:

    • Pathogenic Leptospira serotypes grew on urea, with activity correlating to concentration.
    • Urease activity was detected in pathogenic strains but not saprophytic strains.
    • Urease activity was enhanced in disrupted pathogenic cells and absent when grown on ammonium chloride.

    Conclusions:

    • Leptospira possess urease activity, particularly pathogenic strains.
    • Urease likely contributes to the kidney tropism and pathology of Leptospira infections.
    • Urea utilization is a significant factor in Leptospira pathogenesis.