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Cell surface antigen induced by Friend murine leukemia virus is also in the virion
Abstract:
Intact particles of Friend leukemia virus derived from infectious mouse serum absorb only trace amounts of cytotoxic anti-FMR antibodies, but physical disruption of the virions by freezing and thawing, by ether extraction or by detergent treatment releases large amounts of FMR antigenic activity. Thus this antigen, previously considered to occur mainly as a neo-antigen on the surfaces of virus-infected cells and as a soluble substance in the serum of infected mice, may be primarily a virion component.
Insights
Friend leukemia virus (FMLV) FMR antigen is mainly a component of intact virus particles. Disruption of FMLV virions releases significant FMR antigenic activity, suggesting it is not primarily a cell surface neo-antigen.
Area of Science:
- Virology
- Immunology
- Oncology
Background:
- Friend leukemia virus (FMLV) is associated with FMR antigen.
- FMR antigen was previously thought to be a neo-antigen on infected cells or a soluble serum substance.
Purpose of the Study:
- To investigate the primary location and nature of the FMR antigen associated with Friend leukemia virus.
Main Methods:
- Analysis of FMR antigenic activity in intact and disrupted FMLV particles.
- Disruption methods included freezing/thawing, ether extraction, and detergent treatment.
Main Results:
- Intact FMLV particles showed minimal absorption of anti-FMR antibodies.
- Physical disruption of FMLV virions released substantial FMR antigenic activity.
Conclusions:
- The FMR antigen is predominantly a component of the FMLV virion itself.
- This finding challenges the previous understanding of FMR antigen as primarily a cell-associated or soluble antigen.