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Related Experiment Videos

Hepatitis B core antigen: immunology and electron microscopy.

L F Barker, J D Almeida, J H Hoofnagle

    Journal of Virology
    |December 1, 1974
    PubMed
    Summary

    Hepatitis B virus has two main antigens: hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBcAg). Research shows these antigens are distinct components of the infectious Dane particle.

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    Area of Science:

    • Virology
    • Immunology
    • Hepatology

    Background:

    • Hepatitis B virus (HBV) is associated with two primary viral antigens: hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBcAg).
    • HBsAg is found in serum, while HBcAg is located within the liver during infection.

    Purpose of the Study:

    • To differentiate the antigenic and structural properties of HBsAg and HBcAg.
    • To elucidate the composition of the infectious form of Hepatitis B virus, the Dane particle.

    Main Methods:

    • Serological testing using antisera raised against purified HBsAg and HBcAg.
    • Immune electron microscopy to visualize viral components and their interactions.

    Main Results:

    • Antisera against HBsAg reacted with the Dane particle's surface and associated 20-nm particles, but not the core or HBcAg.

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  • Antisera against HBcAg reacted with the Dane particle's internal core and HBcAg particles, but not the surface or HBsAg-associated particles.
  • Purified HBcAg particles are 27 nm and show distinct subunits.

    • Conclusions:

    • The Dane particle, 42 nm in diameter, is the infectious form of HBV.
    • The Dane particle consists of an inner 27-nm nucleocapsid core (HBcAg) and an outer, distinct lipoprotein surface component (HBsAg).