Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium absorption and intestinal calcium-binding protein: quantitative relationship.

J J Feher, R H Wasserman

    The American Journal of Physiology
    |May 1, 1979
    PubMed
    Summary

    Vitamin D influences calcium absorption by affecting calcium-binding protein (CaBP). A new model reveals a nonfunctional CaBP pool, showing absorption is independent of vitamin D status after accounting for it.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Radioisotopes in the study of mineral metabolism.

    Progress in nuclear energy. Series 6 Biological sciences·2014
    Same author

    Binding proteins from animals with possible transport function.

    The Journal of general physiology·2009
    Same author

    Vitamin D and the dual processes of intestinal calcium absorption.

    The Journal of nutrition·2004
    Same author

    Comparative immunolocalization of the plasma membrane calcium pump and calbindin D28K in chicken retina during embryonic development.

    European journal of histochemistry : EJH·2003
    Same author

    Ryanodine-sensitive, thapsigargin-insensitive calcium uptake in rat ventricle homogenates.

    Molecular and cellular biochemistry·1999
    Same author

    The cardiac content of sarcoplasmic reticulum in the rat determined by calcium uptake rate, calcium oxalate capacity, ryanodine binding and thapsigargin titration.

    Journal of molecular and cellular cardiology·1998

    Area of Science:

    • Biochemistry
    • Nutritional Science
    • Molecular Biology

    Background:

    • Vitamin D is crucial for calcium homeostasis.
    • Calcium-binding protein (CaBP) is a key mediator of vitamin D's effects on calcium absorption.
    • The precise relationship between CaBP levels and calcium absorption requires further elucidation.

    Purpose of the Study:

    • To investigate the relationship between vitamin D-induced CaBP concentration and duodenal calcium absorption in chicks.
    • To determine how factors like vitamin D source, dosage, and time affect this relationship.
    • To develop a model for better understanding CaBP's role in calcium absorption.

    Main Methods:

    • Utilized an in vivo ligated-loop technique in chicks to measure duodenal calcium absorption.

    Related Experiment Videos

  • Quantified the concentration of vitamin D-induced calcium-binding protein (CaBP).
  • Developed and applied a phenomenological model to analyze the CaBP-calcium absorption relationship.

    • Main Results:

    • The relationship between CaBP and calcium absorption varied based on vitamin D source, dosage, and time post-administration.
    • A phenomenological model indicated the presence of a 'nonfunctional' CaBP pool.
    • This nonfunctional pool's size is dependent on the animal's vitamin D status.

    Conclusions:

    • After correcting for the nonfunctional CaBP pool, the proportionality between CaBP and calcium absorption is independent of vitamin D status.
    • This finding offers a refined understanding of vitamin D's mechanism in calcium absorption.
    • The developed model provides a framework for interpreting CaBP function in calcium transport.