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B.K. virus haemagglutinin.

M Kende, M Uj, G Szücs

    Acta Microbiologica Academiae Scientiarum Hungaricae
    |January 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    The optimal conditions for B.K. virus haemagglutinin titration involve the HSAG medium at pH 5.75-6.25 and 4°C. Haemagglutinin stability is maintained across a broad pH and temperature range, with specific salt concentrations preventing degradation at 56°C.

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    Area of Science:

    • Virology
    • Biochemistry
    • Immunology

    Background:

    • B.K. virus (BKV) is a human polyomavirus known to cause various infections.
    • Haemagglutinin (HA) is a key viral surface protein involved in host cell attachment and immune recognition.
    • Accurate titration of viral haemagglutinin is crucial for diagnostic and research applications.

    Purpose of the Study:

    • To determine the optimal buffered medium for B.K. virus haemagglutinin titration.
    • To investigate the stability of B.K. virus haemagglutinin under various environmental conditions.
    • To identify conditions that preserve haemagglutinin titre during storage or processing.

    Main Methods:

    • Titration of B.K. virus haemagglutinin using different buffered media, including HSAG (hepes-salt-albumin-gelatin).

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  • Assessment of haemagglutinin titre at varying temperatures (4°C to 56°C).
  • Evaluation of haemagglutinin stability across a range of pH levels (5.5 to 9.5) and NaCl concentrations (0.063 M to 2.56 M).

    • Main Results:

    • The HSAG medium at pH 5.75-6.25 was identified as the most favorable for BKV haemagglutinin titration.
    • Optimal titration temperature was determined to be 4°C.
    • Haemagglutinin remained stable at temperatures up to 37°C, pH 5.5-9.5, and NaCl concentrations of 0.063 M-2.56 M.
    • Incubation at 56°C led to a time- and pH-dependent decrease in titre, which was mitigated by high NaCl molarity (1.31 M-2.56 M).

    Conclusions:

    • The HSAG medium and specific pH range provide optimal conditions for BKV haemagglutinin titration.
    • BKV haemagglutinin exhibits considerable stability under physiological conditions but is sensitive to prolonged heat exposure.
    • High salt concentrations can protect BKV haemagglutinin from thermal degradation, suggesting potential for improved sample preservation techniques.