Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Kinetics of the hemerythrin-oxygen interaction.

D J de Waal, R G Wilkins

    The Journal of Biological Chemistry
    |April 25, 1976
    PubMed
    Summary

    The kinetics of Golfingia gouldii hemerythrin oxygenation and deoxygenation were studied. Hemerythrin behaves as eight independent units, with consistent reaction rates across various conditions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Using a system of diagnosis and treatment planning to reduce stress.

    Compendium of continuing education in dentistry (Jamesburg, N.J. : 1995)·2001
    Same author

    Levels of expression of P-selectin, E-selectin, and intercellular adhesion molecule-1 in coronary atherectomy specimens from patients with stable and unstable angina pectoris.

    The American journal of cardiology·1997
    Same author

    Outcomes research: a definition?

    Nutrition (Burbank, Los Angeles County, Calif.)·1997
    Same author

    Kinetics and thermodynamics of the reaction of deoxyhemerythrin with nitric oxide.

    Acta chemica Scandinavica (Copenhagen, Denmark : 1989)·1989
    Same author

    Hemodynamic and oxygen transport response to modified fluid gelatin in critically ill patients.

    Critical care medicine·1989
    Same author

    Reversal of intractable septic shock with norepinephrine therapy.

    Critical care medicine·1988

    Area of Science:

    • Biochemistry
    • Chemical Kinetics
    • Protein Dynamics

    Background:

    • Hemerythrin is an oxygen-binding protein found in marine invertebrates.
    • Understanding hemerythrin's reaction kinetics is crucial for elucidating its biological function.
    • Previous studies have not fully characterized the kinetic parameters of Golfingia gouldii hemerythrin.

    Purpose of the Study:

    • To determine the kinetic rate constants and activation parameters for the oxygenation and deoxygenation of Golfingia gouldii hemerythrin.
    • To investigate the influence of protein concentration, ionic strength, and pH on these reactions.
    • To assess whether octameric hemerythrin functions as a single unit or as eight independent subunits.

    Main Methods:

    • Stopped-flow spectrophotometry was employed to monitor the rapid reactions.
    • Oxygenation kinetics were measured directly.
    • Deoxygenation kinetics were determined by scavenging released oxygen with sodium dithionite (S2O4(2-)) or myoglobin.

    Main Results:

    • The second-order oxygenation rate constant (k1) was determined to be 7.4 X 10(6) M-1 s-1, with activation parameters deltaH1++ = 8.2 kcal-mol-1 and deltaS1++ = +1 e.u.
    • The first-order deoxygenation rate constant (k-1) was found to be 51 s-1, with activation parameters deltaH-1++ = 20.6 kcal-mol-1 and deltaS-1++ = +19 e.u.
    • Kinetic parameters remained consistent across varied protein concentrations, ionic strengths, and pH ranges, indicating independent behavior of the octameric hemerythrin subunits.

    Conclusions:

    • Golfingia gouldii hemerythrin exhibits simple second-order oxygenation and first-order deoxygenation kinetics.
    • The octameric hemerythrin functions kinetically as eight independent subunits.
    • Activation parameters derived from kinetic data align well with independent thermodynamic measurements, validating the kinetic model.

    Related Experiment Videos