Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

The NBT test in burned patients.

E A Roe, R J Jones

British Journal of Experimental Pathology

|April 1, 1979

Summary

Polymorphonuclear leukocytes, identified by nitro-blue tetrazolium (NBT) staining, significantly increase post-burn injury. These elevated levels, even without infection, may indicate burn severity and prognosis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fracture-related hospitalisations in newly diagnosed high-risk localised or metastatic hormone-sensitive prostate cancer: secondary analysis of the STAMPEDE phase III trials of docetaxel and zoledronic acid using healthcare systems data.

Annals of oncology : official journal of the European Society for Medical Oncology·2025

Same author

Cancer Trial Impact: Understanding Implementation of the Short Course Oncology Treatment (SCOT) Trial Findings in colorectal cancer at a National Level.

Clinical oncology (Royal College of Radiologists (Great Britain))·2022

Same author

Using diffusion MRI data acquired with ultra-high gradient strength to improve tractography in routine-quality data.

NeuroImage·2021

Same author

Fermentative volatile fatty acid production and recovery from grass using a novel combination of solids separation, pervaporation, and electrodialysis technologies.

Bioresource technology·2021

Same author

Corrigendum to Addition of docetaxel to hormonal therapy in low- and high-burden metastatic hormone sensitive prostate cancer: long-term survival results from the STAMPEDE trial: Ann Oncol 2019; 30: 1992-2003.

Annals of oncology : official journal of the European Society for Medical Oncology·2020

Same author

Addition of docetaxel to hormonal therapy in low- and high-burden metastatic hormone sensitive prostate cancer: long-term survival results from the STAMPEDE trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2019

Area of Science:

Immunology
Burn Medicine
Cellular Biology

Background:

Burn injuries trigger complex inflammatory responses.
Polymorphonuclear leukocytes play a crucial role in the early inflammatory phase.
Nitro-blue tetrazolium (NBT) staining is a functional assay for activated phagocytes.

Purpose of the Study:

To investigate the dynamic changes in NBT-positive polymorphonuclear leukocytes following burn injury.
To correlate NBT-positive cell counts with burn severity and patient outcomes.

Main Methods:

Quantification of NBT-positive polymorphonuclear leukocytes in peripheral blood samples.
Serial blood sampling at various time points post-burn.
Assessment of burn surface area and presence of infection.

Main Results:

A sharp increase in NBT-positive polymorphs was observed within the first week post-burn, reaching 4-5 times baseline levels.
A secondary, smaller increase occurred 4-6 weeks after in burns exceeding 20% body surface area.
Elevated NBT-positive polymorph levels were independent of burn infection.
A fatal case of septicaemia showed persistently low NBT-positive polymorph counts prior to death.

Conclusions:

NBT-positive polymorphonuclear leukocytes are a sensitive indicator of the acute inflammatory response to burn injury.
The pattern of NBT-positive cells may reflect burn severity and potentially serve as a prognostic marker.
Further research is warranted to explore the clinical utility of NBT assay in burn management.

Related Experiment Videos