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Cable properties and compartmentation in Acetabularia.

C Freudling, D Gradmann

    Biochimica Et Biophysica Acta
    |April 4, 1979
    PubMed
    Summary
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    Investigating Acetabularia cell compartments revealed that electrical properties change significantly with cell structure. The study concludes the electrogenic chloride pump is in the plasma membrane, not the vacuole.

    Area of Science:

    • Cell biology
    • Electrophysiology
    • Biophysics

    Background:

    • Acetabularia cells possess unique electrical cable properties.
    • Intracellular compartmentation, particularly vacuole presence, can influence cell electrical behavior.
    • Understanding membrane properties is crucial for cell function.

    Purpose of the Study:

    • To investigate the electrical cable properties of Acetabularia cells with varying intracellular compartmentation.
    • To determine the location of the electrogenic chloride pump.
    • To correlate intracellular structure with electrical membrane parameters.

    Main Methods:

    • Centrifugation to create normal cells, stumps (no central vacuole), and tubes (cytoplasm-depleted vacuoles).
    • Measurement of voltage responses to rectangular current pulses.

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  • Analysis of electrical parameters including conductance, apparent capacity, and resting potential.

    • Main Results:

    • All cell types exhibited a biphasic voltage response.
    • Stumps showed increased conductance and apparent capacity, with a more negative resting potential.
    • Tubes displayed decreased conductance and apparent capacity, with a more positive resting potential compared to normal cells.

    Conclusions:

    • The electrogenic chloride pump in Acetabularia is located in the plasmalemma membrane.
    • The large apparent capacity in stumps is not due to the vacuolar system.
    • Intracellular compartmentation significantly impacts electrical membrane parameters.