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Published on: October 2, 2012
Mutations altering the catalytic activity of a plant-type ribulose biphosphate carboxylase/oxygenase in Alcaligenes
Abstract:
Use of a bacterial system has allowed the isolation of several mutationally altered species of ribulose-1,5-bisphosphate carboxylase/oxygenase having a wide range of catalytic activities. Biochemical and serological techniques were used to classify the mutant strains into three groups: (1) no detectable ribulose-1,5-bisphosphate carboxylase/oxygenase protein present (both subunits missing); (2) mutant strains having ribulose-1,5-bisphosphate carboxylase/oxygenases with lowered specific activities; and (3) mutant strains producing large quantities of catalytically inactive ribulose-1,5-bisphosphate carboxylase/oxygenase.
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