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Transmembrane potentials in guinea-pig hepatocytes.

P Heller, W Van der Kloot

    The Journal of Physiology
    |December 1, 1974
    PubMed
    Summary
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    Guinea-pig liver cells exhibit a resting potential (E) significantly different from potassium equilibrium. Ion concentration changes have minimal impact on E, suggesting complex underlying mechanisms for liver cell membrane potential.

    Area of Science:

    • Cellular Physiology
    • Membrane Electrophysiology
    • Hepatocyte Function

    Background:

    • The resting potential of liver cells is crucial for cellular function but not fully understood.
    • Previous studies suggest a significant deviation from the potassium equilibrium potential in hepatocytes.

    Purpose of the Study:

    • To investigate the determinants of the resting potential (E) in guinea-pig liver cells.
    • To explore the influence of extracellular ion concentrations on hepatocyte resting potential.
    • To refine electrophysiological models for non-excitable cells.

    Main Methods:

    • Measurement of resting potential in isolated guinea-pig liver pieces.
    • Systematic variation of extracellular potassium, sodium, chloride, hydrogen, calcium, and magnesium concentrations.

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  • Application of the constant field equation (Brading & Caldwell, 1971) for data interpretation.

    • Main Results:

    • Guinea-pig liver resting potential (E) averaged -49.8 mV, higher than rat liver but lower than K+ equilibrium potential.
    • E showed minimal sensitivity to extracellular potassium ([K+]o) changes below 20 mM and limited depolarization even at high [K+]o.
    • Variations in other ions ([Na+]o, [Cl-]o, [H+]o, [Ca2+]o, [Mg2+]o) had negligible effects on E.

    Conclusions:

    • Hepatocyte resting potential is not solely determined by potassium gradients, unlike in many excitable cells.
    • The permeability ratios P(Na)/P(K) < 0.04 and P(Cl)/P(K) ≈ 0.3 were estimated, with additional undetermined factors influencing E.
    • Further research is needed to elucidate the physical basis of these additional factors and apply the model to other cell types.